TMEM175 Knockout cell line （SH-SY5Y ）

TMEM175 Knockout cell line （SH-SY5Y ） Order

Catalog#

YKO-H907

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TMEM175 Knockout cell line (SH-SY5Y )

Morphology

Epithelial-like, adherent

Passage ratio

1:2-1:3

Species

Human

Gene Name

TMEM175

Gene ID

84286

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 10 (based on transcript TMEM175-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SH-SY5Y			STR Info (Cell bank) Cell Line:SH-SY5Y
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	11		11	11
D7S820	7	10		7	10
D16S539	8	13		8	13
VWA	14	18		14	18
TH01	7	10		7	10
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	11		11	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM175 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM175 gene knockout TMEM175 Knockout cell line (SH-SY5Y ) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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